Comparative study of physiochemical analysis of fresh and. From this it concluded that the honey pff variety was the best than the remaining. Beth mole, ars technica, transplanting poop can be beneficialswapping vaginal fluids may be even better, 27 sep. All honey samples were prepared using the acetolysis method erdtman 1952, louveaux et al. The reference values for 144 honey samples of different botanical origin were determined by classical physical and chemical methods. Quantitative analysis of physical and chemical measurands in honey by midinfrared spectrometry abstract fourier transform infrared spectroscopy ftir was used to determine 20 different measurands in honey. The physicochemical properties of honey samples from umuahia in abia state, nigeria were compared with those of samples obtained from other towns obudu and nsukka in the region with the aim of observing the differences between these samples and how they differ from an imported brand of honey galeys pure honey produced by nestle uk ltd. Physicochemical analysis and determining of toxic elements in honey produced in the region of tiaret. Keeping this in view physicochemical and minerals determination as quality parameters of fresh floral pakistani honeys produced by a. Physicochemical, heavy metals and phenolic compounds analysis of libyan honey samples collected from benghazi during 20092010 34. Honey samples were divided into two groups based on the mean values of color lightness l fig. Unfortunately due to large market profit for honey sales, adulteration is extremely widespread. Quantitative analysis of physical and chemical measurands in. Antioxidant activities of all honey samples were performed by using percentage inhibition of dpph free radical, aeac, and frap.
Normally, honeys are classified as monofloral, when the pollen frequency of one plant is over 45%. Seven samples of honey were obtained namely from sample a forestry honey ibadan, sample b pure honey, sample c mr. The antioxidant capacity of honey samples was examined using in vitro assays. Physicochemical properties and pollen analyzes of some. Physicochemical properties of honey produced in sekota. Doner, research leader and research chemist, respectively, science and education administration, eastern regional research center, philadelphia, pa.
Recent examples on the web researchers next did tests for active infections and past exposures, probed the genetics of vaginal microbes, and looked at the physicochemical properties of vaginal fluids. Physicochemical properties of stingless bee honey from. Phytochemical analysis and chemical composition of different. Comparative study of the physicochemical and palynological. The samples were submitted to both a qualitative and a quantitative analysis. The physicochemical characteristics of honey, such as high viscosity and density, consistency and sweetness, are due to the fact that it is actually a solution with a high concentration of sugars. Comparative analysis of physicochemical properties of honey from ecological zones and branded honey of pakistan ammara blouch1, rashid mahmood1, khalid rafique1, farid asif shaheen4, masooma munir2, aqsa qayyum2, rabia ali3 1honeybee research institute, national agricultural research centre narc, islamabad, pakistan.
Physicochemical analysis of honey samples collected from local markets of rawalpindi and islamabad, pakistan article pdf available april 2014 with 4,798 reads how we measure reads. Ensuring your honey is pure using nmrbased screening for quality and authenticity analysis with food authenticity of growing concern, honey is one of the foods under increasing scrutiny and its production is now subject to a number of regulations designed to ensure authenticity and quality. Physicochemical analysis article about physicochemical. The results of fresh honey indicated average means of color as 48. Honey is defined as the natural sweet substance produced by apis mellifera bees from the nectar of plants or from secretions of living parts of plants or excretions of plantsucking insects on the living parts of plants, which the bees collect, transform by combining with specific substances of their own, deposit, dehydrate, store and leave in. The present study was carried out to determine the quality of honey samples with local and imported brands available in the rawalpindi and islamabad markets. Ozler, h 2015 melissopalynological analysis of honey samples belonging to different districts of sinop, turkey. In addtion, the hygroscopic nature of honey its tendency to absorb water is detrimental toward bacteria or fungal growth because this causes dehydration. Since, there is no enough scientific published information about the quality and characteristic properties of libyan honey. The comb honey samples were kept under suitable storage conditions until filtration. After the mechanical filtration, the samples were stored until analysis in a jar at room temperature.
Honey, physicochemical, antioxidant activity, dpph, minerals introduction the composition, flavour and colour of honey vary considerably depending on its botanical source. Worth knowing information about honey honey analysis. Honey is acidic in nature, with a ph ranging from 3. Melissopalynological analysis of honey samples belonging to. This natural phenomenon happens when glucose, one of three main sugars in honey, spontaneously precipitates out of the supersaturated honey solution. The samples were stored in a black cupboard at room temperature until needed for the analysis.
Physicochemical analysis of apis dorsata honey from terai forests, nepal samina qamer, farooq ahmad, farooq latif, syed shahid ali and. For all chromatographic separations, a perkinelmer altus. Phytochemical analysis and chemical composition of. Examination of the honey samples revealed that only sample 8 had no pollen content and according to the codex alimentarius, no pollen should be removed because pollen is an essential tool in the analysis of honey. Improvement of mead fermentation by honeymust supplementation pdf. Pollen analysis of honey, or melissopalynol ogy, is of great importance for quality control. Analysis of biochemical composition of honey samples from. Vivian ngoi although honey is already known to possess nutritious health benefits, still many researchers are interested to investigate the ways to process honey without affecting its beneficial properties. Physicochemical analysis and sugar profiling of acacia honey. It is useful as a guide to choosing appropriate method for analysis, classification and authentication of honey.
Retail honey has always been subjected to some form of processing during honey production thus its crucial to assess its quality periodically so as to ascertain whether it satisfies the minimum set requirements. Physicochemical properties and estimation of mineral. Physical and chemical screening of honey samples available. With the honey analysis different tests with honigen are accomplished, which are consulted particularly for the quality regulation. Dealt in the following one with research methods at the national institute for bee customer to be accomplished.
Melissopalynological analysis of honey samples showed a wide variability between samples from different honey geographical origins. The physicochemical properties of honey samples from umuahia in abia state, nigeria were compared with those of samples obtained from other towns obudu and nsukka in the region with the aim of observing the differences between these samples and how. The physical parameters include the mean moisture content of mustard, litchi and nigella honey. Study on the physicochemical parameters, phenolic profile and. Techniques for the evaluation of physicochemical quality and. From homogenous to honey by bryan talbot and neil gaiman. Physiochemical, biochemical, minerals content analysis, and. Correlation study of honey regarding their physicochemical. Comparative analysis of physicochemical properties of honey. The variables analyzed were ph, % purity, ash content, refractive index, viscosity, colour and specific gravity. Discrimination of natural mature acacia honey based on.
Honey is a natural sweet substance produced by bees apis mellifera. Species of pollen are generally used to indicate the floral nectar sources utilized by bees to produce honey. Eyobel mulugeta, wodaje addis, lemessa benti, miresa tadese, physicochemical characterization and pesticide residue analysis of honey produced in west shewa zone, oromia region, ethiopia, american journal of applied chemistry. Pdf physicochemical properties, minerals, trace elements. Honey is a natural sweet substance produced by honey bees, from the nectars of plant flowers and honey dew. Physicochemical characteristics of honey from different.
Beekeeping in the united states agriculture handbook number 335 revised october 1980 pages 82 91. Physicochemical analysis a method of investigating physicochemical systems that makes possible a determination of the nature of the interactions between the components of a system through a study of the relations between the systems physical properties and composition. Physicalchemical parameters of honey of stingless bee hymenoptera. Honey 1 honey 5 and honey 4 were classified into the first group, while the second group comprised the remaining honey types. Ayurvedic as well as yunani medicine have been using honey as a vital medicine for centuries. Study of physicochemical parameters and antioxidant in honey. Pdf volatile profile and physicochemical analysis of. A materials and methods ten samples of honey were randomly collected form different areas of ibadan, oyo state, nigeria. The method consists in the acid treatment of the honey sample, followed by sediment washing and centrifugation.
Honey is one of the most popular natural sweet substances. The results show that honey treatments result in a much greater sterility of the wounds, a faster rate of healing, and a faster onset of healing. Physicochemical parameters affected the primary production in different areas. Honey crystallizes because it is a supersaturated solution. Honey is composed of different chemical compounds, including sugars, proteins, amino acids, vitamins, organic acids, phenolic compounds and volatile compounds. Pdf physicochemical analysis and antibacterial activity. When the moisture % contents of these honey samples are observed can be divided in three groups. Physicochemical analysis of soil collected from babhulgaon.
Sample collection and analysis thirty fresh honey samples of different botanical sources and geographical regions of the country were collected from farm gates following their harvested seasons. Harmonized methods of melissopalynology ihc international. Department of biochemistry, faculty of science, university of maiduguri, nigeria. Zolkapli1 1 department of pharmacology and chemistry, faculty of pharmacy, universiti teknologi mara uitm, malaysia. Five standard physicochemical parameters were determined according to the international legislation. Physicochemical definition is being physical and chemical. The physicochemical analysis of sixteen honey samples of local brand is shown in table 3 and 4. Honey is produced in most of the countries of the world. Through processes such as the specific enzymes present in honey, high temperature and long storage time, honey may change and degrade into new products such as furans, amino acids, alcohols and new phenolic compounds and new volatile. Physicochemical characteristics and microbiological.
Effect of processing treatment on antioxidant, physicochemical and enzymatic properties of honey trigona spp. Honey ph has been useful in the determination its geographical origin acquarone et al. Effect of fructose on body weight in controlled feeding trials. The qualitative and quantitative screening test for phytochemicals such as tannins, phlobatanins, flavonoids, terpenoids, glycosie, saponins, alkaloids, flourides of branded and unbranded honey samples were carried out by official methods of analysis 12. The homogenous comic is a collaboration between bryan talbot and neil gaiman with inks by mark buckingham and letters by steve craddock it was a protest against proposed homophobic legislation in the uk in 1988 and showed what would happen if all fey and homosexual influences were removed from society.
International journal of basic and applied research. Sesame sesamum indicum the frequent grain is the main source of nectar 41% followed by the buckthorn rhamnus sp. Honey it is found that a mixture of honey and cinnamon cures most of the diseases. In group one, there were significant differences between honey 1 and honeys 5 and 4 while in group two. It has been reported that honey isomaltose, laminaribiose, nigerose, kojibiose, gentiobiose samples showed regional variation in the physicochemical and btrehalose. European legislation 1 defines various honey types and. This study has examined the impacts of soil physicochemical pdf reader virus properties ph, total organic. Dec 14, 2014 this is a project for class 12 boards for chemistry subject on analysis of honey. Protein contents and physicochemical properties in honey. Major components of honey analysis by nearinfrared. The determination was based on the method proposed by ferreira et al. Honeycombs were also stored at the refrigerator for analysis. The pollen analysis was performed using the acetolysis method.
Most moisture values of all samples were in concordance to the set values of the b ureau of indian standards bis, 2002 with lowest of. It has great diversity, giving it a multitude of properties, both nutritionally and therapeutically. Volatile profile and physicochemical analysis of acacia. The physicochemical of chemical characteristics of water samples in the study area suggested that there was no harmful chemical contamination. The methods of sampling and analysis to be employed for the determination of the. The study revealed that all honeys had ph, water content. Honey is a kind of directly consumed natural product, produced by honeybees apis mellifera l. Physicochemical and microbiological properties of honey. Honey is a popular natural food product with a very complex composition mainly consisting of both organic and inorganic constituents. Honey sometimes takes on a semisolid state known as crystallized or granulated honey. Physicalchemical parameters of honey of stingless bee. Toma4 1valahia university of targoviste, faculty of sciences and arts, 082 targoviste, romania, eu email.
A study on the determination of physicochemical properties of. From a chemical point of view, it could be defined as a natural food mainly composed of sugars and water together with minor constituent such as minerals, vitamins, amino acids, organic acids, flavonoids and other phenolic compounds and aromatic substances. Research centre assisted by performing analyses to detect honey. Pollen analysis in honey samples from the two main producing. Honey is described in codex alimentarius standard and in turkish food codex as a naturally sweet product produced. The samples of honey were analysed according to the aoac methods in order to determine moisture, optical rotation, acidity free, lactone and total and ash content. Analysis of biochemical composition of honey samples from northeast nigeria. Physicochemical, heavy metals and phenolic compounds. Chemical composition of honey university of bristol.
This type of physicochemical and pollen analysis of honey samples favors the possibility of utilizing the rich flora of western ghats for good quality honey. Honey is a sweet, viscous food substance made by honey bees and some related insects. This study aimed to study the physicochemical and microbiological characteristics of honeys collected during the dry and rainy seasons in the different phytogeographical areas of benin. The statistical models of the chromatic determination, physicochemical parameters and volatile profile was optimal to characterize the honey samples and group them into three geographical origins, even they belong to the same botanical origin. The sweetness of honey offsets the acidic nature of honey. Honey can be characterized according to its identified includes maltose, sucrose, maltulose, turanose, geographical origin. The composition of honey is strongly influenced by both natural and anthropogenic factors, which vary based on its. Physicochemical characteristics, antioxidant activities and phenolic profile of honey obtained from extrafloral nectar efh and the honey obtained from a mixed floral source wild honey were compared in this study. Table 8 gives a comparison of relevant published data of honey parameters from different regions of india and some other countries of indian subcontinent to the present study. Physicochemical properties, antioxidant features of honey. Estevinho3 1 pharmaceutical science school, university of sao paulo, av. The analysis results of physicochemical parameters of honey belonging to various floral origins are presented in table 7.
The present study aimed to evaluate physicochemical characteristics and quality of honey. Physicochemical characterization and pesticide residue. The aim of the current study was the comparative analysis of the physical, biochemical and antioxidant properties of mustard, litchi, and nigella honey. Honey is a remedy for every illness and the quran is a remedy for all illness of the mind, therefore i recommend to you remedies, the quran and the honey bukhari. Introduction honey is a carbohydrate rich naturally complex product. Overall, the ph of stingless bee honey ranges from 3. Honey was used from ancient time both as a natural sweetener and a healing agent national honey board, 2002. Physicochemical definition of physicochemical by merriam. Physicochemical analysis and sugar profiling of acacia honey s.
Physicochemical characteristics and pollen spectra of organic. Physicochemical analysis of honey samples collected from local markets of rawalpindi and islamabad, pakistan. Multifloral honey, physicochemical characteristics, pollen analysis, unifloral honey, western ghats. Honey quality can also be affected by heating during. This study indicated the results of analysis of the reliable and. Nguyen 2 1 department of apiculture and sericulture, university of. Physicochemical analysis all honey samples had acceptable values of speci. The healing properties of honey were demonstrated in a study comparing honey treatment to that of silver sulfadiazine, the standard treatment, for burn victims. The principles of physicochemical analysis were established in the late 19th.
An attempt to determine major quality markers of honey article pdf available in bulletin of the chemical society of ethiopia 261. This study intended to determine and compare the microscopic and physicochemical characteristics of burkina fasan honey n 27 with those described in the codex standard, and to also find correlations between individual constituents. Tiaret, algeria, report by advances in environmental biology. Original article comparative study of the physicochemical and palynological characteristics of honey from melipona subnitida and apis mellifera ligia b. Honey market is a large part of the economy for many countries. The pollen analysis is a great tool that may be used to detect organic honey because it helps to identify the plants visited by honeybees. Determination of the botanical origin of honeybee honeys. Honey is variable in its composition, due to contribution of the plant, climate, environmental conditions and the ability of the beekeeper white, 1978. According to melissopalynological analysis of honey samples, the examined honey samples were considered as natural bee honey, while, pollen analysis indicated that, kashmiri and saudi honey might be produced from bee colonies fed partially with sugar syrup abd alla et al. Physicochemical analysis of honey samples collected from.
Pdf physicochemical analysis of honey samples collected. Analysis of honey chemistry science fair project experiments, chemistry models, exhibition ideas, expo topics for kids and also organics chemistry science ideas for cbse, icse, middleschool, elementary school for 5th, 6th, 7th, 8th, 9th and high school students. Environmental issues electrical conductivity analysis chemical properties electric properties honey pollution influence. Hplc system was used, including the altus a10 solvent and. Pdf physicochemical analysis of honey in the kenyan. Abstract based on pollen analysis, honey samples of 21 different localities in boyabat, duragan, erfelek, gerze, sarayduzu and turkeli districts of sinop were investigated. Its quality and nutritional value is of great importance for consumers. Microscopical analysis, especially the identification and quantification of pollen grains in honey sediment, is the reference method used to determine the botanical origin of sample honeys.
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